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Numerous studies reported that symptomatic and asymptomatic bacteriuria is common in pregnant women. It may be related with serious obstetric complications. Therefore, the current study aimed to finding the prevalence of bacteriuria, bacterial profile and antibiotic resistance pattern of uropathogens. A total of 140 pregnant women were participated in this study from November 2015 to February 2016. The result revealed that the overall prevalence of bacteriuria was 47.14%, while prevalence of bacteriuria with symptomatic and asymptomatic were (43.28%) and (50.68%) respectively, which shows that there was not statistically significant differences between the two studied groups. The pregnant women with age between 15-19 years had the highest rate of bacteriuria (85.71%). The high frequent bacteriuria also was found in third trimester (56.25%) compared with first (30.43%) and second (43.40%). Escherichia coli (22.73%), Staphylococcus aureus (19.7%), and Staphylococcus epidermidis (10.61%) were the predominant isolated uropathogens. Antibacterial susceptibility test was achieved for all isolated strains by the Kirby-Bauer’s disk-diffusion method. Our result revealed that more than 90% of the isolates were resistant to cephalexin and amoxicillin/clavulanic acid. However amikacin was the most potent of all studied antibiotic 77.3% of uropathogens were sensitive to it. The resistance rates to ≥3 antimicrobial agents was 98.5% while, only 1.5% were resist to all antimicrobial tested. (1)