انتاج إنزیم الھیمولایسین Hemolysin من العزلة المحلیة لبكتریا cholerae Vibrio المعزولة من مرضى مصابین بالإسھال

Abstract

Diagnosed 26 isolate of vibrio cholera was isolated from clinical sources responsible of causing diarrhea. All isolates showed high productivity of hemolysin by different efficiency. Semi quantitative screening on a selective solid medium (blood ager additive 7% blood) showed that all isolates have the ability to produce hemolysin as indicated by the formation of clear zone around the colonies. The selected isolate characterized as Vibrio cholera AMK6 based on its high production of enzyme and used in the present study. The optimum conditions for hemolysin production by submerged cultures (brain heart infusion containing 3%Glycerol) with an inoculums size of 3x108 CFU at an initial pH of 8 with shaking incubation at 35°C, 150 cycle/min for 24 hours.