PRODUCTION OF PECTINASE FROM ASPERGILLUS NIGER BY USING SOME FRUIT PEELS

Abstract

Ten filamentous fungi isolated from a growaste samples .Three isolated were of high productivity for pectinase enzyme compared with other isolates , depending on diameter of clear hydrolyzed zones on the medium plates containing commercial pectin as sole carbon source , this isolates were Aspergillusniger (A 2 ),closely followed byAspergillus sp.(A 1 ) and penicillum sp.(P 1 ) . The three isolates also produced pectinases with different a growastes ( Orange peels , Apple peels , Banana peels)as the sole carbon source ,Aspergillusniger(A 2 ) was the best isolate for pectinase production on the medium containing orange peels as the sole carbon source . Peak pectinase activity of 675 and 370 u/mg protein was respectively obtained by solid – state fermentation (ssf) and submerged fermentation (smf) for A.niger(A 2 ) .Solid – state fermentation yielded higher levels of pectinase activity than the submerged fermentation . The strain of A.niger(A 2 ) have good prospect for pectinase production ,and the orange peels is a good low – cost fermentation substrate for pectinase production by the investigated isolate