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http://148.72.244.84:8080/xmlui/handle/xmlui/10975| Title: | EXTRACTION AND PURIFICATION OF URASE FROM Proteus vulgaris Pv12 ISOLATED FROM CHILDREN INFECTED WITH URINARY TRACT INFECTION IN IRAQ |
| Authors: | Hadi R.R. Al–Taai |
| Keywords: | Proteus vulgaris , urease , gel filtration , molecular weight , Isoelectric point |
| Issue Date: | Jun-2011 |
| Publisher: | University of Diyala / College of Agriculture |
| Citation: | https://journal.djas.uodiyala.edu.iq/ |
| Series/Report no.: | Vol. 3;No. 1 |
| Abstract: | The results of purification of urease was extracted from local isolates Proteus vulgaris Pv12 showed the specific activity 33.33 unit / mg protein by ammonium sulfate with ( 25- 50 ) % percentage of saturated , and specific activity 109 . 68 unit / mg protein , purified 60.03 fold and 55.3% of yield by DEAE sepharose , While the results indicated the specific activity 175.09 of unit / mg protein , purified 90.25 fold and 36.55% of yield by sephacyl S- 200chromatograghieles . Using buffer (PEM) 20mM With 7.5 pH. Molecular weight of enzyme was about 215000 Dalton determined by gel filtration. The results indicated the isoelectric point of purified urease that extracted from Proteus vulgaris Pv12 was 5.3. |
| URI: | https://journal.djas.uodiyala.edu.iq/ http://148.72.244.84:8080/xmlui/handle/xmlui/10975 |
| ISSN: | ISSN: 2310-8746 (Online) ISSN: 2073-9524 (Print) |
| Appears in Collections: | مجلة ديالى للعلوم الزراعية / Diyala Agricultural Sciences Journal (DASJ) |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| EXTRACTION AND PURIFICATION OF URASE FROM Proteus vulgaris Pv12 ISOLATED FROM CHILDREN.pdf | 258.46 kB | Adobe PDF | View/Open |
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