WATERY EXTRACT AND METHONOLIC EXTRACTION OF NERIUM OLEANDER EFFECT ON MICROTUBULES OFH22 CELL AND PROGRAMMED CELL DEATH .

Abstract

The study evaluated the effect of selected Oleandrin and methonolic extraction of Nerium oleander on the microtubule network of H22 cells line (hepatic cell) GFP tubulin labeled . Cells were treated with watery extract and methonolic extraction at various concentration from 0.01 μ/ml to 10m/ml for 60 min , , microtubules were detected by mean of indirect immunfluorescence. the damage was examined in afluorescnce microscope . Therapeutic effect of both extracts was studied in tumor-bearing mice after (I.P) administration of watery extract at concentrations of methonolic (16.5 , 8.1, 4.1mg/kg) and methonoloic extract at concentrations (20, 10, 5 mg/kg) for 30 days alternatively. The results revealed significant reduction in the tumor volume weather in those treated with watery extract 84.09%, or those treated with alcoholic extract 86.07%, particularly at the concentration of 16.5 mg/kg and 20 mg/kg respectively .