Histopathological Changes of Serratia Marcescens In Mice

Abstract

A prominent opportunistic pathogen that affects a range of hosts, including invertebrates, plants, and mammals, has been identified as the cause of Serratia marcescens, a gram-negative bacillus belonging to the Enterobacteriaceae family. Numerous clinical conditions are associated with it, such as meningitis, pneumonia, keratitis, urinary tract infections, and wound infections; Given that its red colonies are easily identifiable, Serratia marcescens is utilized as a biological marker. The current research sought to investigate the alterations in mice immunized against Serratia marcescens infection with (WCSA-S) of S. marcescens and (KWCA-S) of S. marcescens. Twelve male albino mice were split up randomly into three groups (4 mice for each group) and their histopathological changes examination was performed. The first group was vaccinated with WCSAg-S (500 μg/ml) vaccine subcutaneously (S/C). The third group (the negative control) was given PBS (1 ml) subcutaneously, while the second group received KWCA-S (9 x 108cfu/ml) vaccinations (pH 7.2). After 14 days, the male albino mice were given booster doses of the same antigens.. after 28 days of immunized groups with infective dose (1x106 cfu/ml) of Serratia marcescens in order to determine the histopathological changes in the internal organs (liver, spleen, kidneys, lungs, and intestine) that showed severe histopathological changes in the positive control groups compared with other immunized groups.