Clinical Evaluation For The Wet Mount Preparations Versus Ziehl–Neelsen Staining Modifications For Diagnosis And Severity Scoring Of Cryptosporidium Parvum In Children Under 5 Years

Abstract

Aim : Current study aims to investigate the Clinical Evaluation for the wet mount preparations versus Ziehl–Neelsen staining modifications for Diagnosis of cryptosporidium parvum in children under 5 years in Baqubah-Diyala province Methods : fecal samples were collected from (100)diarrheic children and stained by Lugol's iodine solu-tion; concentration using Sheather's Sugar solution ;Ziehl–Neelsen staining (ZN) ,cold &hot techniques and Kinyoun’s staining method for detection of C. parvum Results: both of sheather's sugar solution concentration technique and ZN hot staining were identical in diagno-sis of negative C. parvum among diarrhea cases in children . Significant difference as well as correlation regarding the detection and scoring of C. parvum oocysts among children was reported between sheather's sugar concentration and ZN hot staining procedure (P value=0.000).Very good agreement (kappa =0.832, p value= 0.000),was reported between ZN hot staining procedure and sheather's sugar concentration technique for diagnosis of C. parvum oocysts in children . both of lugol's iodine technique and ZN hot staining were identical in diagnosis of negative C. parvum among diarrhea cases in children . Significant difference as well as correlation regarding the detection and scoring of C. parvum oocysts among children was reported between lugol's iodine and ZN hot stain-ing procedure (P value=0.000).Very good agreement (kappa =0.659, p value= 0.000),was reported be-tween ZN hot staining procedure and lugol's iodine for diagnosis of C. parvum oocysts in children . Conclusion : Wet mount preparations (lugol's iodine and sheather's sugar concentration technique consid-ered as a very good alternative to ZN hot staining procedure for diagnosis of C. parvum oocysts among children in primary health care centres and hospitals.