Clinical Agreements Between Ziehl Neelsen And Methylene Blue Staining Modifications For Detection Of C.parvum Infection In Claves

Abstract

Aim : To investigate the agreements between Ziehl Neelsen and Safranin Methylene Blue Staining mo-dalities staining techniques for detection of C.parvum infection in calves during first year of life in Diyala province Methods :Fifty diarrheic calves under 1 year were included .stool samples were examined after stained with Ziehl –Neelsen ;Safranin Methylene Blue Staining modalities Results: Both of ZN hot stains and ZN cold , Kinyoun's technique ; Safranin Methelyne Blue (SMB) and Modified Safranin Methelyne Blue (Modified SMB) were identical in diagnosis of (34%), negative C.parvum among diarrhea cases. A total of (20%) have low score ; (22%) moderate scores and (8%) heavy score in both techniques. Good agreement (kappa =0.780),was reported between ZN hot staining procedure and ZN cold technique for diagnosis of C.parvum oocysts in calves .A total of (24%) , have low score ,(8%) moderate and (4%) with heavy score in both techniques (Hot ZN & Kinyoun's tech-nique). Moderate agreement (kappa =0. 586),was reported between ZN hot staining and Kinyoun's tech-nique for diagnosis of C.parvum oocysts in calves. A total of (24%) have low score,(14%) moderate and (8%) with heavy score in both techniques(ZN&SMB). Good agreement (kappa =0. 699),was reported between ZN hot staining procedure and SMB technique for diagnosis of C.parvum oocysts in calves. A total of (20%) have low score,(18%) moderate and (8%) with heavy score in both techniques. Good agreement (kappa = 0.726),was reported between ZN hot staining procedure and Modified SMB technique for diagnosis of C.parvum oocysts . Conclusions : All Alternative stains for Hot ZN( ZN cold ; Kinyoun's, SMB and modified SMB staining have identical specificity (100%) for diagnosis of negative C.parvum among diarrhea cases in calves . According to good agreement between ZN hot and( ZN cold ; SMB , Modified SMB ) ,these stains can be used as first line alternative to hot ZN stain in diagnostic laboratory .While Kinyoun's staining technique used as a second alternatives due to moderate agreement with ZN hot staining for diagnosis of C.parvum oocysts in calves which represent good alternatives in rural areas and low income countries