Bacteriological and Molecular Study of Escherichia coli Isolated from Urinary Catheters in Baquba Teaching Hospital

Abstract

One Hundred fifty-two sample of catheters and urinary tracts from patients in the Urology Unit at Baquba Teaching Hospital for the period from 15/7/2018 to 30/9/2018 for different age groups ranging from 1-80 years for both sexes, were collected. Fifteen isolates from Escherichia coli were subjected to microscopic and biochemical tests and the diagnosis was confirmed by using the Vitek 2 compact system the results of the detection of some virulence factors of the selected E. coli. The percentage of the ability of the bacteria to produce hemolycin enzyme was 53.33%, The ability of the isolates to form the biofilm was detected by Congo Red method and it was 40%. Resistance of the selected isolates to 7 antibiotics which belong to the Beta lactam group including Cefoxitin, Amoxicillin, Ephalexin, Ceftazidiume, Augmentin, Cefotaxime and Impinem. The ratios of antibiotic resistance were 86.7%, 73.3%, 60%, 73.3%,40%, 40% and 66.7%, respectively.

The poly chain reaction (PCR) was conducted for the detection of DNA of (8) E.coli isolates that showed resistance to the beta lactam antibiotics using primers of the genes bla SHV, bla CTX-M and bla TEM. The results showed that all isolates were 100% found carriers of these genes.