Virulence Factors of Acinetobacter baumannii isolated from different clinical specimens in Baquba

Abstract

The study included isolation and identification of 16 isolates of Acinetobacter baumannii out of 196 samples collected from different clinical specimens in Baquba Teaching Hospital and Al-Batool Teaching Hospital from 1/10/2014 to 16/2/2015. The highest isolation rate of these bacteria from wounds and burns was (10.8% , 8.3%) respectively , and urine was 6.9% , the blood culture were to contain less proportion of these bacteria 5% . The diagnosis of isolates was based on phenotypic , microscopic characteristics and biochemical tests , in addition to use ViTEK2 device to confirm the diagnosis. The results of the investigation of virulence factors of Acinetobacter baumanniishowed that all isolates have the ability to adhere on surfaces of epithelial cells of humans (100%), while the ability of 13 isolates to produce biofilm was 81.2% , while Seven isolates had possessed efflux pumps with high efficiency (43.7%) .The investigation of the sensitivity test against4 antibiotics from B-lactam group , the results showed the highest percentage was resistant to antibiotic Cephalexin 100% and lowest resistance to antibiotic Imipenem 50% .It was determined the Minimum Inhibitory Concentration MIC for cefotaximeand ceftazidime by method of multiplying serial concentrations , the results inducted that the minimum inhibitory concentration for cefotaxime values ranging between 32-1024 µg/ml , as for the MIC ceftazidime his values ranging between 16-1024 µg/ ml.