Bacteriological Characterization OF Some Gram Negative Bacteria Isolated From Vaginal Infections

Abstract

This study included collection 410 samples of vaginal swabs from patients woman's in hospitals of Baquba city for the period from 1/9/2015 to 31/12/2015.The results showed that the highest percentage of vaginitis in pregnant woman(65%) than in both nonpregnant and nonfruitl (30%,5%) respectively The results refer that (24%)Escherichia coli, (20%) Proteus mirabilis by using diagnostic phenotypic ,biochemical test and conifirm the diagnosis using VITEC 2 analyser The results of the investigation of some virulence factores showed the isolstes of Escherichia coli and Proteus mirabilis produce of haemolysin with percentage (50%) and(90%) respectively. The results showed that all isolates of Proteus mirabilis were produced urease by(100%),while the results detect that the isolates of Escherichia. coli is not able to produce this enzyme. The results showed that isolates of Proteus mirabilis produced protease by (45%), while Escherichia coli have not ability to produce this enzyme. Results showed that all isolates of Escherichia coli have ability to adhesion surface of epithelial cells of human by(100%),while the isolates Proteus mirabilis showed ability to adhesion by(85%).The minimum inhibitory concentration MIC for 6 antibiotics, that Ampicillin, Amoxicillin ,Amekacin ,Cefotaxime,Gentamycin ,Tetracycllin was determined .The MIC values of these antibiotics ranged between (512-1025≥),(32-1024≥),(4-64),(8-1024),(4-128),(4- 512)µ\ml,respectively.The minimum inhibitory concentration MIC for disinfectant povidone iodine10%, chloroxyzylenol 3%(50,100,200,300,400,500)µ\ ml,respectively.Results Combination antibiotic Gentamycin and disinfectant povidone iodine10% reduced the ability of isolates for adherence.The results showed averiance as far their resistance to these antibiotics of Escherichia coli and Proteus mirabilis showed highest resistance rate 100% for Ampicillin and Amoxicillin.