Studying the Some biochemical parameters and Molecular genetic study for thalassemia patients in Diyala province

Abstract

The present study aimed to study some Biochemical parameters in thalassemia patients in Diyala province , The study included 72 patients major beta thalassemia during the period from September until may of 2016, were the age between 1.5 to 32 years , as well as 32 healthy person with out genetic blood disease and were between the ages of 3 to 25 year , The study results showed a significant increase in the rate of biochemical parameters if there is a significant increase in the enzyme liver function ALT the concentration in patients was : 23.74 ± 29.53 U\L and in healthy was 4.61 ± 11.67 U\L , also there was an increase in the creatinine concentration patients 9.92 ± 26.08 mmol\L and in healthy was, 68.06 ± 51.54 mmol\L , the urea concentration was a decrease in of patients 0.89 ± 3.96 mmol\L and in healthy was 1.03 ± 2.36 mmol\L, there was an increase in iron concentration in patients 382.05 ±64.37 Umol\L And in healthy was 9.48 ± 61.36 Umol\L, Decrease glucose concentration in patients and healthy height , 18.58 ± 28.59 Umol\L9.10 ± 20.44 Umol\L respectively .the genetic molecular study show that been isolated DNA chromosomal of the blood of persons with the disease and blood healthy persons using several extraction Genetic DNA mini kit blood protocol the purity of DNA extracted between ( 1.8 – 2 ) and the use of one specialist to detect mutation raster the : IVS.I.5 (G-C) , using Amplification Refractory Mutation System (ARMS – PCR ) .