Inhibition of Mitotic Index and Proliferation of Cell Line H22 by Alkaloids Extraction from Isatis tinctoria

Abstract

Alkaloids extraction from Isatis tinctoria inhibited H22 cell line (hepatic cell line ) proliferation by inducing mitotic index at the metaphase/ anaphase boundary. Halfmaximal inhibition of cell proliferation occurred at 8 nM alkaloids, and mitosis was halfmaximally block 8 nM alkaloids. Formation of an incomplete metaphase plate of chromosomes was associated with inhibition of mitosis and an altered arrangement of spindle microtubules that strongly resembled the organization abnormal that occurs with little concentrations of alkaloids extraction of plant and other antimitotic compounds. No increase in spindle microtubule occurred below 10 nM alkaloids extract. The results indicate that alkaloids shares a common antiproliferative mechanism with extraction alkaloids . The mass of spindle microtubules increased half-maximally at 80 nM alkaloids and attained maximal levels at 33 nM alkaloids extract .At submicromolar concentrations, bovine brain tubulin in a manner that resemble suppression suppressed growing and shortening by extract alkaloids for Isatis tinctoria at the ends of microtubules reassembled in vitro. The alkaloids was concentrated in H22 cells several hundredfold. At its lowest effective concentrations, alkaloids appears to block mitosis by stabilizing spindle microtubules kinetically and not by changing the mass of microtubules polymerized