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DC Field | Value | Language |
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dc.contributor.author | Mohammed Ibrahem Nader | - |
dc.contributor.author | Amer Hani Razaq AL.Shammary | - |
dc.contributor.author | Muthanna Abdulkhader Al-Mahdawi | - |
dc.date.accessioned | 2023-11-08T08:27:41Z | - |
dc.date.available | 2023-11-08T08:27:41Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 2222-8373 | - |
dc.identifier.uri | http://148.72.244.84:8080/xmlui/handle/xmlui/8615 | - |
dc.description.abstract | The Hemolysin was Extracted from Vibrio cholera cultured on Brain Heart infusion by cold centrifugation 5000 r/min for 15 min at 4°C, and purified by several steps, including precipitation with 75% saturation ammonium sulphate, ion exchange chromatography using DEAE-cellulose and gel filtration on Sephadex G-100 column. The obtained purification foldand recovery were 24.26 unit /ml and 32.47% respectively. The molecular weight of the enzyme is about 19490 Dalton as determined by gel filtration. The optimum pH for activity and stability was 6 and 6-8 respectively. The enzyme retained its original activity when incubated at 20-45oC for 15 min, the activity of the enzyme decreased after enzyme incubation at 45oC. The maximum enzyme activity observed at 30oC. | en_US |
dc.description.sponsorship | https://djps.uodiyala.edu.iq/ | en_US |
dc.language.iso | en | en_US |
dc.publisher | university of Diyala | en_US |
dc.subject | Vibrio cholera, haemolysin purification, haemolysin characterization | en_US |
dc.title | Purification and characterization of Hemolysin produced by local isolates of Vibrio cholera | en_US |
dc.type | Article | en_US |
Appears in Collections: | مجلة ديالى للعلوم الاكاديمية / Academic Science Journal (Acad. Sci. J.) |
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