Bacterial infection of Diabetic foot ulcer

Abstract

The study included 25 wound samples were collected from type 1 insulin dependent diabetes mellitus (IDDM), and type2 non-insulin dependent diabetes mellitus (NIDDM) foot patients admitted to daquq hospital of both sex and their ages between 41-75 years. The study aimed to screen the aerobic bacterial pathogens present in diabetic pus and to determine their antibiotic susceptibility against common standard antibiotics. Bacteriological diagnosis and antibiotic sensitivity profiles were carried out at two parts: The profile part swab was taken has been cultured in media of blood agar and MacConkey agar depend on biochemical tests and indicators. The results indicate that common pathogen isolates from the diabetic pus included E. coli followed by Proteus mirabilis and Staphylococcus aureus, and the peak proportion of diabetic foot ulcer (DFU) was recorded between 41-75 years in both sexes. The second step were included the performing antibiotic susceptibility according to Kirby-Bauer disc diffusion method on Mueller–Hinton agar using 11 different antibiotics. It was obvious that Nitrofurantoin, Ciprofloxacin and rifampicin is more active against E. coli followed by co-trimoxazole and gentamycin . Ofloxacin is more active against Staphylococcus aureus followed by gentamycin and Amipcillin .Amikacin, Nitrofurantoin and Co-Trimoxazole is active against Proteus mirabilis followed by gentamycin and erythromycin.