Abstract:
This study was designed to explore the pathological and prophylactic effect
of the mix of Dandelion ethanolic extract with the bacterium lactobacillus
acidophilus in vitro and in vivo using laboratory mice. Dandelion ethanolic
extract, lactobacillus acidophilus and the mix of Dandelion ethanolic extract
with the bacterium lactobacillus acidophilus were dealing in in vitro study
against (Salmonella typhimurium, Pseudomonas aerogenosa
, Staphylococcus
aureus and Escherichia coli) using the concentration 100 mg/L for Dandelion
extract, also dealing with the virulent bacteria in equal quantity and separately.
Lactobacillus acidophilus had the highest inhibitory effect on the Pseudomonas
aerogenosa, and the less inhibitory effect was by Dandelion extract, and the
inhibitory effect of Dandelion extract on the pathogenic bacteria increased after
mixed with lactobacillus acidophilus bacteria. Twenty four mice were randomly
divided into six groups, each ghroup contain four animals. The first group
infected with Pseudomonas aerogenosa and the dose 1x106 cfu orally for 48
hours. The second group infected with Pseudomonas aerogenosa with dose
1x106 cfu orally for two weeks. The third group infected with Pseudomonas
aerogenosa and the dose 1x106 cfu orally for 48 hours, they treated with the mix
of Dandelion ethanolic extract and lactobacillus acidophilus orally (0.3
ml/mice) for two weeks. The fourth group were treated with the mix of
Dandelion ethanolic extract and lactobacillus acidophilus orally (0.3 ml/mice)
for two weeks, and then infected with Pseudomonas aerogenosa and the dose
1x106 cfu orally for 48 hours. The fifth group administered Dandelion extract
and concentration 100 mg/ml (0.3 ml/mice) orally for two weeks. The sixth
group administered 0.3 ml/mice of normal saline as control group. The
histopathological study showed pathological changes in the internal organs of
the first and second groups that infected with Pseudomonas aerogenosa
bacteria. The mix of Dandelion extract and lactobacillus acidophilus apparently
has therapeutic effect more than prophylactic effect on the inhibition of
Pseudomonas aerogenosa growth.
