Abstract:
The study was included isolation and identification of bacterial strains
resistant of pyrethoid pesticides (lambde-cyhalothrin, fenvalerate)from
agricultural soils in Baquba city. The results appeared that the most of bacteria
strain are Pseudomonas aeruginosa .All bacterial isolates were identified by the
biochemical, cultural and microbial characteristic and then isolated adapted
to mineral salt media containing pesticides .The research findings of
residue concentration by use HPLC technique that Pseudomonas
aeruginosa was adapted and growth to concentration Viz. 250μg/ml , 200 μg
/ml of (lambde-cyhalothrin, fenvalerate).
The plasmid content of the local isolates was studied and the results showed
isolates contain single large plasmid Band, curing was conducted by use of
Acridin orange. The plasmids were lost at concentration 1024μg/ml and then
isolated adapted to concentration 200μg/ml lambde-cyhalothrin
thereafter the culture was assessed by HPLC and results showed the rate of
residue concentration in last week was 100 ,it was concluded that the major
gene for pesticides degradation was located on plasmid.