الخلاصة:
Antimicrobial activity of oil, aqueous and alcoholic extracts of anise
seeds against gram positive (Bacillus subtilis , Staphylococcus aureus )and
gram negative (Escherichia coli , Pseudomonas aeruginosa)bacteria and molds
(Rhizopus spp
, Mucor spp ,Aspergillus spp ,Penicillium spp) were tested ;those
microorganisms represent common causes to some human and animal employed
in extracting anise oil. Concentration of (1000,2000,4000 PPM)of the extracts
above were used to find their action in the tested microorganisms. It has been
shown that Ba-subtiles and Staph. aureus bacteria were affected more than
Escherichia coli , Pseudomonas aeruginosa .The maximum diameters of
inhibitory zones (24, 26 mm) were appeared at a concentration of 4000 ppm of
oil extract against the tested gram positive bacteria comparing with 13 ,18 mm
of diameters of inhibitory zones recorded against the tasted gram negative
bacteria at the same conditions. The tested mold Rhizopus spp was the most
susceptible to the oil extract, the percentages of their inhibition at constrictions
of 2000 and 4000 ppm were 30 and 40% consequently in comparison with
alcoholic extract 95% which was 7.4 and 8.8% for Bacillus subtilis ,
Staphylococcus aureus consequently .The alcoholic extract had no effect in the
growth of Escherichia coli even at the concentration of 4000 ppm. The molds
Rhizopus spp
, Mucor spp were influenced more than ,Aspergillus spp
,Penicillium spp since the percentages of inhibition were 29,28,25,22%
consequently. The aqueous extract was not effective in all tested
microorganisms because it had no anise oil and thus no anithol dissolved (the
active material),generally result indicated from seed Anise oil have more
activity than alcoholic and water extracts.
