Immunohistochemical Expression of Antioxidant Enzyme PRDX6 in Oral Squamous Cell Carcinoma In Relation to Clinicopathological Parameters

Abstract

Background:The PRDX6 antioxidant family is important in the protection of cells against oxidants in humans. It is encoded by the PRDX6 gene. Peroxiredoxin 6 antioxidant enzyme (PRDX 6) has been reported in several cancers. However, the function of PRDX 6 in oral squamous cell carcinoma (OSCC),is far from being understood. Objective:To normal corresponding oral mucosa in relation to clinicopathological parameters. Patients and Methods: This study included 40 formalin-fixed, paraffin-embedded tissue blocks of primary OSCC cases and fifteen normal oral mucosa samples. Demographic data, clinical staging and histopathological grading were obtained. Immunohistochemically staining anti PRDX 6 antibody was done to illustrate its subcellular localization in normal and malignant cells. Results: Females were affected more than males and the tongue was the most common site involved. PRDX 6 expression was high in OSCC than normal (p=0.001) with mean±Sd (60±18.8) (23±9.8). Significant correlations were found with tumor size, lymph node and tumor stage (p=0.008, p=0.012, p=0.011 respectively) . Whereas no relation was observed concerning age, gender and tumor site. Conclusion: Significant correlation of PRXD 6 anti oxidant enzyme expression with tumor size, lymph node involvement and clinical staging, suggesting its role in oral squamous cell carcinoma progression.