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Title: | Spectrophotometric Determination of Tetracycline Hydrochloride Through Coupling with Sulphanilic Acid |
Authors: | Rasul J Ali Hawar J Sadiq Hawezy Mohammad S Abdullah |
Keywords: | Spectrophotometry Tetracycline Azo-coupling |
Issue Date: | ديس-2018 |
Publisher: | University of Diyala - College of Medicine |
Abstract: | Background: Tetracycline is a broad-spectrum antibiotic that is used to treat diseases caused by different types of bacteria. Objective:To estimate tetracycline hydrochloride in pharmaceuticals through coupling with a new reagent, sulfanilic acid. Patients and Methods: The proposed method for determination of tetracycline hydrochloride was applied to four different types of pharmaceutical drug companies and the results were compared with a standard method (HPLC method) to determine their compatibility and the results were satisfactory. Results: The method is based on azo coupling reaction between sulfanilic acid and tetracycline in alkaline medium. The colored compound produced by the reaction has a higher absorbance at a wavelength of 403nm. The Beer ,s law was applied in the concentration range (8.0 - 95.0μg / ml) and the detection limit, molar absorptivity and correlation coefficient were 0.2μg / ml, 6.536x103L / mol.cm and 0.9997 respectively. The method was successfully applied to the evaluation of tetracycline hydrochloride in pharmaceuticals. Conclusion:The proposed method for determination of tetracycline hydrochloride is a simple and sensitive method, where the tetracycline hydrochloride can be estimated at low concentration and accurately. This method needs a simple device and the method can be used in quality control centers. The recommended method has been successfully applied to estimate tetracycline hydrochloride in pharmaceuticals. |
URI: | https://djm.uodiyala.edu.iq/index.php/djm http://148.72.244.84:8080/xmlui/handle/xmlui/3546 |
ISSN: | Print ISSN 2219-9764 Online ISSN 2617-8982 |
Appears in Collections: | مجلة ديالى الطبية / Diyala Journal of Medicine |
Files in This Item:
File | Description | Size | Format | |
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3-Mohammad S. Abdullah3.pdf | 687.11 kB | Adobe PDF | View/Open |
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