Pathogenicity Study of a number of Gram- negative bacilli isolated from clinical samples

Abstract

Fifty isolates from different clinical samples (urine and wounds) were collected from AlNu'man General Hospital in Baghdad city. Sampling activates were carried out from 23April to 23June 2014. The age of patient range between 40- 70 years . The Gram-negative isolates were subjected to antibiotic susceptibility, β-lactamase production (86%), biofilm formation (84%), hemolysin production (48%).The resalt appered that Escherichia coli (54%) was the most common followed by Proteus vulgaris (18%), Enterobacter aerogenes (8%), Proteus mirabilis (6%), Enterobacter cloacae (4%), Acinetobacter baumannii (4%) and (2%) for each Klebsiella oxytoca, Enterobacter gergaviae and Pseudomonas mendocina. Gram negative isolates were observed resistance (100%) for Ampicillin, Carbenicillin, Cefalothine, Cefotaxim and Meropenem. Most isolated bicteria showed less resistance to Amoxicillin / clavulanic acid and Ceftazidime, also they showed sensitivity to Imipenem. Plasmid profile analysis, Proteus vulgaris isolates showed one large plasmid only.