PATHOLOGICAL STUDY AND GENETIC DIAGNOSIS OF VINBLASTINE EFFECTS ON DNA OF OVARY AND TESTES IN MOUSE

Abstract

The present study was designed in order to identify the effects of vinblastine on ovary and testes and then on fertilization capacity and pregnancy outcome in white mice, fifty mixed mice were used, 10 treated male were mated with 10 untreated female, 10 untreated male were mated with 10 treated female, and (10) mixed mice as control. Results of male test showed decline in sperm count with spermatocytes' morphological deformity. The results of molecular technique applied on testicular section and ovarian section, showed that green signals, indicating the damage on TK (11qE2)/XY gene on chromosome of sperm and ova, red signal indicate intact gene yellowish signals indicated translocation on the same gene (mutation). Pathological findings showed marked loss of spermatogenesis (Azoospermia) with damage in testes' tissues; fibrosis of ovarian stroma with degenerative changes on ovary and evidence of early embryonic death with absence or low fertility.