STUDYING OF IMMUNE RESPONCE OF MICE IMMUNIZED BY Rhizopus ANTIGENES

Abstract

Zygomycosis is an infection causes by different fungi, Rhizopus spp are one of these causative fungi that ubiquitous in nature and have worldwide distribution. To find out the immune response against Rhizopus spp in this study two antigens were prepared from pathogenic Rhizopus spp. The first one was spore antigen which extracted from spores and the second was mycelium antigen from fungal mycelium. These antigens were used in experimental animals (30 mice) which were divided to three groups. The first group was immunized by spore antigen and the second group with mycelium antigen. The third group was a control. Immunological tests were performed for all tested groups including the delayed type hypersensitivity test to detect the cellular immunity. The results showed that the mean values of skin thickness in immunized mice with spore antigen at 24 hours post examination, in the 1st group were (1.7±0.07) while in the 2nd group were (2.4±0.14), and these values declined at 48 hours post- examination in the 1st group (1.1±0.04) and the 2nd group (1.7±0.06). The humeral immunity detected by Enzyme-linked Immunosorbent Assay (ELISA), the results showed that immunized animals with mycelium antigen express high levels in the means concentration of (IgG) (270.5±29.55) in the second group when compared with the first one (132.2±11.45). In the control group, (IgG) remained with normal range in all mice serum, was (8.1±1.3).