استخدام صبغة الكونغو الحمراء في الوسط الزرعي لتسريع عزل وتنقية وتشخيص الأنواع التابعة لجنس Azospirillum

Abstract

151 isolates of Azospirillum were identified out of 289 nitrogen fixing bacteria, these isolates were obtained from soil rhizosphere and sterilized rice roots of different regions in Iraq, by using biochemical and physiological characters, the isolates were differentiated into three species namely: A. brasilense, A. Lipoferum, and A. irakense and their numbers were 88,16 and 47 respectively. All isolates of A. irakense (except two) were obtained from sterilized root. By using modified Red Congo medium for isolation, purification and identification. The isolates were classified into three groups (A, B and C) according to colony colors. The results indicated that there is a relationship between biochemical characters and textures, shapes , colors of the colonies. It seems that there is a similarity between colonies of the same group, which differ from the other groups.