الخلاصة:
In this study , one hundred and fifteen clinical samples were collected from
patient suffering from a variety of infections. Number of collected samples and
types of infections were distributed as follows , Fifty one from wounds and
burns infections , twenty eight from (UTI) infections , eleven from eye
infections and twenty-five from ear infections .The initial identification , which
included morphological features on culture media were done at the hospital site
, and demonstrated that forty three 43 isolates were diagnosed as
Staphylococcus spp
.Twenty four isolates (from this forty three isolates) isolates
were diagnosed as S. aureus. All the isolates (43) were tested for the cultural
examination at the site of isolation and biochemical tests were performed , and
the use of the system (Api20) in order to be diagnosed until species. The results
showed that (24) from (43) isolates were diagnosed as Staphylococcus aureus.
The results showed that (13) from (24) isolates were collected from wounds and
burns infections, (5) from (24) isolates from (UTIs) , (4) from (24) isolates from
Ear infections , and (2) from (24) isolates were collected from Eyes infections.
All isolates were tested for methicillin antibiotics to determine the resistance
and sensitive isolates for this antibiotics, the results showed that (21) from (24)
isolates (87.5%) were resistant to methicillin and (3) from (24) isolates (12.5%)
were sensitive for the same antibiotics. Six type of amino acids were used to
study the impact or effects on the formation of (Biofilm) in bacteria and the amino acids are (Valin , Cysteine , Tryptophan , Phenylalanine , Tyrosine and
Methionine) , the following concentrations of amino acids were used in the
current study (0mM , 50mM , 100mM and 500mM ) , the results showed that
the rate of inhibition increases with the concentration of amino acids in the
media and it prevent the biofilm formation with the gradually and proportional
relationship .
